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Aubergine protein interactors in 0-2 hour-Drosophila embryos

ABSTRACT: To identify Aub function in mRNA regulation in the Drosophila embryo, we have performed mass spectrometry analysis of Aub interactors, following immunoprecipitation of GFP-Aub in 0-2 hour-embryos. Immunoprecipitation of GFP alone was used as negative control. Because Aub accumulates at high levels in the germ plasm, GFP-Aub immunoprecipitation was also performed in oskar mutant embryos that do not assemble the germ plasm. Proteins coprecipitating with GFP-Aub were similar in wild-type and oskar mutant embryos. Translation factors were enriched among proteins coprecipitating with Aub.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Oana Vigy

LAB HEAD: Martine Simonelig

PROVIDER: PXD016399 | Pride | 2020-04-27

REPOSITORIES: Pride

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The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm.

Ramat Anne A Garcia-Silva Maria-Rosa MR Jahan Camille C Naït-Saïdi Rima R Dufourt Jérémy J Garret Céline C Chartier Aymeric A Cremaschi Julie J Patel Vipul V Decourcelle Mathilde M Bastide Amandine A Juge François F Simonelig Martine M

Cell research 20200304 5

Piwi-interacting RNAs (piRNAs) and PIWI proteins are essential in germ cells to repress transposons and regulate mRNAs. In Drosophila, piRNAs bound to the PIWI protein Aubergine (Aub) are transferred maternally to the embryo and regulate maternal mRNA stability through two opposite roles. They target mRNAs by incomplete base pairing, leading to their destabilization in the soma and stabilization in the germ plasm. Here, we report a function of Aub in translation. Aub is required for translationa ...[more]

PMID: 32132673

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