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Prevotella bryantii B14 proteins from in vitro cultivation under various short-chain fatty acids (SCFA)

ABSTRACT: P. bryantii B14 cells were cultivated separately in acetic (Acet), propionic (Prop), butyric (But), iso-butyric (iBut), valeric (Val), iso-valeric (iVal) and 2-methyl butyric acid (2MB) as well as in a mixture of all mentioned short-chain fatty acids (Mix). All 8 treatments were analyzed regarding their proteomes in order to understand the requirements and effects of each SCFA on the metabolism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacteria Prevotella Bryantii B14

SUBMITTER: Jana Seifert

LAB HEAD: Porf. Jana Seifert

PROVIDER: PXD016407 | Pride | 2021-09-09

REPOSITORIES: Pride

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Short-Chain Fatty Acids Modulate Metabolic Pathways and Membrane Lipids in Prevotella bryantii B14.

Trautmann Andrej A Schleicher Lena L Deusch Simon S Gätgens Jochem J Steuber Julia J Seifert Jana J

Proteomes 20201016 4

Short-chain fatty acids (SCFAs) are bacterial products that are known to be used as energy sources in eukaryotic hosts, whereas their role in the metabolism of intestinal microbes is rarely explored. In the present study, acetic, propionic, butyric, isobutyric, valeric, and isovaleric acid, respectively, were added to a newly defined medium containing Prevotella bryantii B14 cells. After 8 h and 24 h, optical density, pH and SCFA concentrations were measured. Long-chain fatty a ...[more]

PMID: 33081314

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