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In-depth proteome profiling of kidney in 5/6 Nephrectomy rat model

ABSTRACT: Chronic kidney disease (CKD) is characterized by a slow and gradual loss of kidney function, with glomerular filtration loss over months or years, inevitably leading to end-stage renal disease. The renal failure resulting from this irreversible process derives from fibrotic lesions of each compartment of the kidney; glomerulosclerosis, vascular sclerosis, and tubulointerstitial fibrosis. Here, we aimed to specify CKD-related injury markers through proteomics analysis in animal kidney tissues.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

DISEASE(S): Chronic Kidney Disease

SUBMITTER: Dohyun Han

LAB HEAD: Dohyun Han

PROVIDER: PXD016447 | Pride | 2021-01-19

REPOSITORIES: Pride

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Multisample Mass Spectrometry-Based Approach for Discovering Injury Markers in Chronic Kidney Disease.

Kim Ji Eun JE Han Dohyun D Jeong Jin Seon JS Moon Jong Joo JJ Moon Hyun Kyung HK Lee Sunhwa S Kim Yong Chul YC Yoo Kyung Don KD Lee Jae Wook JW Kim Dong Ki DK Kwon Young Joo YJ Kim Yon Su YS Yang Seung Hee SH

Molecular & cellular proteomics : MCP 20210113

Urinary proteomics studies have primarily focused on identifying markers of chronic kidney disease (CKD) progression. Here, we aimed to determine urinary markers of CKD renal parenchymal injury through proteomics analysis in animal kidney tissues and cells and in the urine of patients with CKD. Label-free quantitative proteomics analysis based on liquid chromatography-tandem mass spectrometry was performed on urine samples obtained from 6 normal controls and 9, 11, and 10 patients with CKD stage ...[more]

PMID: 33453410

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Quantitative proteomic analysis of hypoxia induced primary kidney cells

Project description:Chronic kidney disease (CKD) is characterized by a slow and gradual loss of kidney function, with glomerular filtration loss over months or years, inevitably leading to end-stage renal disease. The renal failure resulting from this irreversible process derives from fibrotic lesions of each compartment of the kidney; glomerulosclerosis, vascular sclerosis, and tubulointerstitial fibrosis. Nevertheless, despite numerous research efforts, both the definitive mechanism underlying the progression from CKD to end stage renal disease and an effective treatment have remained elusive. In this study, We utilized TMT-multiplexed quantitative proteomics approaches to identify protein expression changes associated with chronic injury in primary cultured renal cells.

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