Dataset Information

Bone morphogenetic protein 4 in human saliva during orthodontic therapy

ABSTRACT: In orthodontic therapy periodontal tissue responds to mechanical stimuli by bone remodeling mediated by specific molecules involved in periodontal regeneration and homeostasis. The resulting changes are reflected in the salivary proteome that is emerging as a valuable diagnostic tool. We analyzed the changes of saliva proteome during orthodontic tooth movement in 12 healthy male patients, presented with malocclusion treated by placement of fixed orthodontic appliance. Six patients with identical dental pathology, but no orthodontic therapy were used as control samples. Saliva was collected a day before, immediately after, and 2, 7 and 30 days after the placement of the fixed orthodontic appliance and analyzed by LC-MS. Total of 198 proteins were identified and classified on the basis of their functional characteristics. Proteins involved in bone remodeling, inflammation and healing were detected mostly 30 days after placement of the fixed orthodontic appliance. At this time point, Bone morphogenetic protein 4 emerged as a central player in the ongoing dental bone remodeling. Besides BMP4, BMP antagonists: BMP Binding Endothelial Regulator (BMPER), Insulin-like growth factor-binding protein 3 (IGFBP3), Cytoskeleton-associated protein 4 (CKAP4) and Fibroblast growth factor 5 (FGF5), were also identified. Presence of BMP4 in human saliva 30 days after the placement of orthodontic fixed appliance was confirmed by ELISA and concentration of BMP4 was 3.2 pg/ml. To date, there is no published data on the presence of BMP molecules or their antagonists in saliva or the gingival cervical fluid, regardless of different orthodontic treatments or disease. BMP4 is involved not only in orthodontic treatment, but also in processes involving tooth development and homeostasis. Therefore, a better understanding of its networks in bone remodeling during OTM is heralded.

INSTRUMENT(S): LTQ Orbitap Discovery

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Ruđer Novak

LAB HEAD: Lovorka Grgurević

PROVIDER: PXD016481 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance.

Grgurevic Lovorka L Novak Ruder R Salai Grgur G Trkulja Vladimir V Hamzic Lejla Ferhatovic LF Popovic Vojka Zgombic VZ Bozic Darko D

Progress in orthodontics 20210712 1

<h4>Background</h4>This study was conducted in order to explore the effects of orthodontic tooth movement (OTM) on the changes of salivary proteome. This prospective observational pilot study recruited 12 healthy teenage boys with malocclusion treated with a fixed orthodontic appliance and 6 appropriate control participants. Saliva samples were collected a day before and at 0, 2, 7, and 30 days after initialization of treatment, corresponding to the initial, lag, and post-lag phases of OTM. Pool ...[more]

PMID: 34250561

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Dataset Information

Bone morphogenetic protein 4 in human saliva during orthodontic therapy

Publications

Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance.

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