Dataset Information

Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow

ABSTRACT: Parasite-derived extracellular vesicles (EVs) are now at the forefront of helminth research, with evidence indicating that they exhibit immune modulatory activity, presenting a strong case to look at their potential as vaccine candidates. In the present study, Fasciola hepatica derived EVs (FhEVs) were isolated from adult worms using a gravity flow method. These gravity flow derived FhEVs were characterised by proteomics and lectin array. Comparative analysis using the same methods was carried out using FhEVs recovered by differential centrifugation. The immunomodulatory properties of the gravity flow derived FhEVs was determined using bone marrow derived murine dendritic cells (BMDCs), which highlighted that the FhEVs induce a novel phenotype that secreted low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L and SIGNR1) and intracellular markers (SOCS1 and SOCS3). In conclusion, this study demonstrates that FhEVs modulate immune cells, inducing a unique DC phenotype that supresses IL-2 secretion from T-cells.

INSTRUMENT(S): LTQ Orbitrap, TripleTOF 5600

ORGANISM(S): Fasciola Hepatica (liver Fluke)

SUBMITTER: Krystyna Cwiklinski

LAB HEAD: John P Dalton

PROVIDER: PXD016561 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.

Murphy Anna A Cwiklinski Krystyna K Lalor Richard R O'Connell Barry B Robinson Mark W MW Gerlach Jared J Joshi Lokesh L Kilcoyne Michelle M Dalton John P JP O'Neill Sandra M SM

PLoS neglected tropical diseases 20200908 9

Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host. In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity. The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by gly ...[more]

PMID: 32898175

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Dataset Information

Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow

Publications

Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.

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