Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion, Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Brain
SUBMITTER: Eva Griesser
LAB HEAD: Angus I. Lamond
PROVIDER: PXD016563 | Pride | 2020-03-05
REPOSITORIES: Pride
Griesser Eva E Wyatt Hannah H Ten Have Sara S Stierstorfer Birgit B Lenter Martin M Lamond Angus I AI
Molecular & cellular proteomics : MCP 20200304 5
Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In combination with formalin-fixed and paraffin-embedded (FFPE) tissue it provides a powerful tool for retrospective and clinically relevant studies of tissue proteins in a healthy and diseased context. We first optimized the protocol for efficient LCM analysis of FFPE tissue specimens. The use of SDS containing extraction buffer in c ...[more]