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Expression of proteins by a marine Brevibacterium casei #NIOSBA88 in response to Arsenic and Chromium

ABSTRACT: A marine bacterial isolate, Brevibacterium casei #NIOSBA88 was grown separately in Luria Bertini broth supplemented with 750 mg L-1 Arsenic (III) and 100 mg L-1 Chromium respectively. Similarly, the strain was grown in absence of Arsenic and Chromium, which served as an experimental control. The cells were harvested at different time intervals and proteins were extracted from the cell pellets using in-house protocol (Shah and Damare 2018). The extracted proteins were digested using trypsin following standard protocol. Tryptic digests were injected in LCMS QToF for obtaining spectral library which was used to identify the proteins against a species specific database in NCBI.

INSTRUMENT(S): 6538 Q-TOF LC/MS

ORGANISM(S): Brevibacterium Casei

TISSUE(S): Prokaryotic Cell

SUBMITTER: Samir Damare

LAB HEAD: Dr. Samir R Damare

PROVIDER: PXD016588 | Pride | 2020-10-21

REPOSITORIES: Pride

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Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium-a proteomic approach.

Shah Shruti S Damare Samir S

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20200729 4

Cellular response against different heavy metal stress differs with the metal. Arsenic and chromium are heavy metals and toxic to living systems. The concentration of these metals in seawater is very low. However, due to their solubility in nature, they actively enter cells via various transport mechanisms and cause damage to the cells. Brevibacterium casei #NIOSBA88, a marine-derived, gram-positive isolate was multi-metal tolerant. Proteomic analysis of this isolate in response to arsenic and c ...[more]

PMID: 32729030

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