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Identification of Arabidopsis NAI2 complex

ABSTRACT: ER bodies are ~10-µm-long, spindle-shaped structures that occur in Brassicaceae plants. They are distributed throughout the epidermal cells of cotyledons and hypocotyls, but subsequently disappear with seedling growth, while they constitutively develop in the epidermal cells of roots. NAI2 is an ER body protein responsible for ER body formation. To understand molecular mechanism of ER body formation by NAI2, we generated transgenic plants that expressed NAI2-GFP under control of the native NAI2 promoter, and conducted immunoprecipitation with anti-GFP antibodies and detected with mass

OTHER RELATED OMICS DATASETS IN: MTBLS1383

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: Kenji Yamada

LAB HEAD: Kenji Yamada

PROVIDER: PXD016606 | Pride | 2020-01-17

REPOSITORIES: Pride

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Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants.

Yamada Kenji K Goto-Yamada Shino S Nakazaki Akiko A Kunieda Tadashi T Kuwata Keiko K Nagano Atsushi J AJ Nishimura Mikio M Hara-Nishimura Ikuko I

Communications biology 20200114 1

Brassicaceae plants have a dual-cell type of chemical defense against herbivory. Here, we show a novel single-cell defense involving endoplasmic reticulum (ER)-derived organelles (ER bodies) and the vacuoles. We identify various glucosinolates as endogenous substrates of the ER-body β-glucosidases BGLU23 and BGLU21. Woodlice strongly prefer to eat seedlings of bglu23 bglu21 or a glucosinolate-deficient mutant over wild-type seedlings, confirming that the β-glucosidases have a role in chemical de ...[more]

PMID: 31937912

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