Proteomics

Dataset Information

0

Thermosensation is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling


ABSTRACT: Organismal physiology and survival are influenced by environmental conditions and linked to protein quality control. Proteome integrity is achieved by maintaining an intricate balance between protein folding and degradation. In Caenorhabditis elegans, acute heat stress determines cell non-autonomous regulation of chaperone levels. However, how the perception of environmental changes, including physiological temperature, affects protein degradation remains largely unexplored. Here, we show that loss-of-function of dyf-1 in Caenorhabditis elegans associated with dysfunctional sensory neurons leads to defects in both temperature perception and thermal adaptation of the ubiquitin/proteasome system centered on thermosensory AFD neurons. Impaired perception of moderate temperature changes worsens ubiquitin-dependent proteolysis in intestinal cells. Brain-gut communication regulating protein turnover is mediated by upregulation of the insulin-like peptide INS-5 and inhibition of the calcineurin-regulated forkhead-box transcription factor DAF-16/FOXO. Our data indicate that perception of ambient temperature and its neuronal integration is critical for the control of proteome integrity in complex organisms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Alexandra Segref  

LAB HEAD: Thorsten Hoppe

PROVIDER: PXD016676 | Pride | 2022-08-12

REPOSITORIES: Pride

altmetric image

Publications

Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling.

Segref Alexandra A   Vakkayil Kavya L KL   Padvitski Tsimafei T   Li Qiaochu Q   Kroef Virginia V   Lormann Jakob J   Körner Lioba L   Finger Fabian F   Hoppe Thorsten T  

Nature communications 20221005 1


Organismal physiology and survival are influenced by environmental conditions and linked to protein quality control. Proteome integrity is achieved by maintaining an intricate balance between protein folding and degradation. In Caenorhabditis elegans, acute heat stress determines cell non-autonomous regulation of chaperone levels. However, how the perception of environmental changes, including physiological temperature, affects protein degradation remains largely unexplored. Here, we show that l  ...[more]

Similar Datasets

2010-03-23 | E-GEOD-21012 | biostudies-arrayexpress
2013-06-01 | E-GEOD-40459 | biostudies-arrayexpress
2018-05-01 | GSE68464 | GEO
2022-10-01 | E-MTAB-11045 | biostudies-arrayexpress
2021-11-25 | E-MTAB-10898 | biostudies-arrayexpress
2012-06-17 | E-GEOD-37433 | biostudies-arrayexpress
2011-12-31 | E-GEOD-24847 | biostudies-arrayexpress
2011-12-31 | E-GEOD-23013 | biostudies-arrayexpress
2016-07-19 | E-GEOD-84489 | biostudies-arrayexpress
2012-02-03 | E-GEOD-35360 | biostudies-arrayexpress