Proteomics

Dataset Information

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NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1

ABSTRACT: We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jack-Ansgar Bruun  

LAB HEAD: Terje Johansen

PROVIDER: PXD016681 | Pride | 2020-01-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
190906_YPA_B10-1.pdResult Other
190906_YPA_B10.raw Raw
190906_YPA_B7-1.pdResult Other
190906_YPA_B7.raw Raw
190906_YPA_B8-1.pdResult Other
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