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Proteomic analysis of APC7 KO mouse Cerebella

ABSTRACT: A proteomic comparison of the cerebella of APC7 KO mice versus controls using TMT11-plex reagents

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebellum

DISEASE(S): Neurodevelopmental Abnormality

SUBMITTER: Brandon Gassaway

LAB HEAD: Steven Gygi

PROVIDER: PXD016683 | Pride | 2023-03-10

REPOSITORIES: Pride

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APC7 mediates ubiquitin signaling in constitutive heterochromatin in the developing mammalian brain.

Ferguson Cole J CJ Urso Olivia O Bodrug Tatyana T Gassaway Brandon M BM Watson Edmond R ER Prabu Jesuraj R JR Lara-Gonzalez Pablo P Martinez-Chacin Raquel C RC Wu Dennis Y DY Brigatti Karlla W KW Puffenberger Erik G EG Taylor Cora M CM Haas-Givler Barbara B Jinks Robert N RN Strauss Kevin A KA Desai Arshad A Gabel Harrison W HW Gygi Steven P SP Schulman Brenda A BA Brown Nicholas G NG Bonni Azad A

Molecular cell 20211222 1

Neurodevelopmental cognitive disorders provide insights into mechanisms of human brain development. Here, we report an intellectual disability syndrome caused by the loss of APC7, a core component of the E3 ubiquitin ligase anaphase promoting complex (APC). In mechanistic studies, we uncover a critical role for APC7 during the recruitment and ubiquitination of APC substrates. In proteomics analyses of the brain from mice harboring the patient-specific APC7 mutation, we identify the chromatin-ass ...[more]

PMID: 34942119

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