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Potential ATF6 regulatory proteins were screened by proteomics using pancreatic tissues of PRSS1 transgenic mice treated with caerulein

ABSTRACT: Humanized PRSS1 transgenic mice were constructed and treated with caerulein to mimic the development of human AP and CP. Potential ATF6 regulatory proteins in pancreatitis were screened by proteomics using pancreatic tissues of PRSS1 AP mice.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Pancreas

DISEASE(S): Pancreatitis

SUBMITTER: Zhang guowei

LAB HEAD: Guo-wei Zhang

PROVIDER: PXD016703 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	19P02970004_DIA.raw	Raw
	19P02970005_DIA.raw	Raw
	19P02970006_DIA.raw	Raw
	19P04740001_DIA.raw	Raw
	19P04740002_DIA.raw	Raw

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Publications

ATF6 aggravates acinar cell apoptosis and injury by regulating p53/AIFM2 transcription in Severe Acute Pancreatitis.

Tan Jie-Hui JH Cao Rong-Chang RC Zhou Lei L Zhou Zhi-Tao ZT Chen Huo-Ji HJ Xu Jia J Chen Xue-Mei XM Jin Yang-Chen YC Lin Jia-Yu JY Zeng Jun-Ling JL Li Shu-Ji SJ Luo Min M Hu Guo-Dong GD Yang Xiao-Bing XB Jin Jin J Zhang Guo-Wei GW

Theranostics 20200709 18

<b>Background:</b> There is no curative therapy for severe acute pancreatitis (SAP) due to poor understanding of its molecular mechanisms. Endoplasmic reticulum (ER) stress is involved in SAP and increased expression of ATF6 has been detected in SAP patients. Here, we aimed to investigate the role of ATF6 in a preclinical SAP mouse model and characterize its regulatory mechanism. <b>Methods:</b> Pancreatic tissues of healthy and SAP patients were collected during surgery. Humanized PRSS1 transge ...[more]

PMID: 32724472

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Potential regulatory proteins in pancreatitis were screened by proteomics using pancreatic tissues of PRSS1 AP mice

Project description:Humanized PRSS1 transgenic mice were constructed and treated with caerulein to mimic the development of human AP and CP. Potential regulatory proteins in pancreatitis were screened by proteomics using pancreatic tissues of PRSS1 AP mice.

2021-09-09 | PXD016651 | Pride

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Proteomic analysis of mice pancreatic islet fed a high fat simple carbohydrate diet

Project description:This study aims to elucidate the molecular mechanisms linking dietary stress from a high-fat simple carbohydrate (HFSC) diet to an elevated risk of pancreatic cancer. Using label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS), we performed a comprehensive proteomic analysis of collagenase-digested pancreatic islets from HFSC-fed mice. The study identified a significant upregulation and downregulation of 33 and 34 proteins respectively in HFSC group compared to the control group. The results revealed substantial alterations in the expression of proteins involved in key biological processes such as metabolic stress, protein synthesis, and endoplasmic reticulum (ER) stress, all of which are crucial for maintaining cellular homeostasis under dietary stress. Notably, the study identified several potential biomarkers, including MANF, VCP, HYOU1, and PRDX1, which are intricately linked to pancreatic function and the progression of cancer. These biomarkers play pivotal roles in managing cellular stress responses, protein folding, and metabolic regulation, offering insights into how a HFSC diet contributes to pancreatic carcinogenesis. This research highlights the significant impact of dietary factors on pancreatic cancer development and underscores the potential of these biomarkers as therapeutic targets for early detection and treatment, ultimately aiming to improve patient outcomes in PDAC.

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Butyrophilin 3A1 contributes to inflammation and induces a lupus-like disease by inhibiting the IL-38-ferroptosis axis

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Gene expresssion changes in pancreatic islets of 11 weeks old IKK2-CApdx-1 mice compared to control and Pdx-1+/- mice

Project description:Canonical IKK/NF-κB signaling is a master regulator of inflammation and innate immunity and has been implicated in the pathogenesis of T1D. To investigate the impact of NF-κB activation on β-cell homeostasis and diabetes development, we generated a transgenic gain-of-function mouse model allowing conditional NF-κB activation via expression of IKK2-CA (constitutively active IKK2 allele) in β-cells using the tetracycline-regulated gene expression system. Pdx-1-tTA (knockin model generating Pdx-1 haploinsufficiency) driver mice were used for β-cell specific transgene expression. Double transgenic IKK2-CA-pdx-1 mice develop a full-blown immune-mediated diabetes.To identify gene expression changes underlying this diabetes development pancreatic islets of diabetic IKK2-CA-Pdx-1, PDX-1 +/- and control mice were prepared and isolated total RNA was used for microarray analysis. Pancreatic islets were isolated from 11 weeks old control (n=5), Pdx-1+/- (n=4) and IKK2-CApdx-1 (n=5) mice, total RNA was extracted with RNeasy mini kit (QIAGEN). Microarray analyses were performed using 200 ng total RNA as starting material and 5.5 µg ssDNA per hybridization (GeneChip Fluidics Station 450; Affymetrix, Santa Clara, CA). The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (http://www.affymetrix.com). Labeled ssDNA was hybridized to Mouse Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA). The chips were scanned with a Affymetrix GeneChip Scanner 3000 and subsequent images analyzed using Affymetrix® Expression Console Software (Affymetrix). Probe level data were obtained using the robust multichip average (RMA) normalization algorithm.

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APPLICATION OF A NOVEL PEPTIDE SPECTRAL LIBRARY USING SWATH ANALYSIS FOR THE QUANTITATION OF PROTEINS IN EX-DIAGNOSTIC CLINICAL SERUM SAMPLES FROM SHEEP

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