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Identifying mTOR regulated RNA binding proteins

ABSTRACT: To investigate the RNA binding proteins that change following mTOR inhibition, HeLa cells were treated with and without 200nM Torin1 for an hour. Cells were the UV254 cross-linked, lysed and large scale whole cell oligo(dT) based pulldowns were performed with identification and quantification of purified proteins by label free mass spectrometry.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Rebekah Jukes-Jones

LAB HEAD: Martin Bushell

PROVIDER: PXD016705 | Pride | 2020-11-24

REPOSITORIES: Pride

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