Proteomics

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Complexome profiling of MICOS complex, impact of subunits MIC26 and MIC27

ABSTRACT: Here we analysed the impact of two subunits (MIC26 and MIC27) on the assembly and stability of the mitochondrial contact site and cristae organizing system (MICOS) complex.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Andreas Reichert

PROVIDER: PXD016732 | Pride | 2020-07-28

REPOSITORIES: Pride

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Publications

Homologous apolipoproteins of MICOS complex, MIC26 and MIC27, show an antagonistic regulation of their protein levels, making it difficult to deduce their individual functions using a single gene deletion. We obtained single and double knockout (DKO) human cells of <i>MIC26</i> and <i>MIC27</i> and found that DKO show more concentric onion-like cristae with loss of CJs than any single deletion indicating overlapping roles in formation of CJs. Using a combination of complexome profiling, STED nan  ...[more]

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