Proteomics

Dataset Information

0

Complexome profiling of mitochondrial protein complexes in single and double MIC26 and MIC27 knockout cells

ABSTRACT: Here we analysed the impact of two subunits of the mitochondrial contact site and cristae organizing system (MICOS) complex, MIC26 and MIC27, on the assembly and stability of mitochondrial complexes of the oxidative phosphorylation system (OXPHOS).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Andreas Reichert

PROVIDER: PXD016733 | Pride | 2020-07-28

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Homologous apolipoproteins of MICOS complex, MIC26 and MIC27, show an antagonistic regulation of their protein levels, making it difficult to deduce their individual functions using a single gene deletion. We obtained single and double knockout (DKO) human cells of <i>MIC26</i> and <i>MIC27</i> and found that DKO show more concentric onion-like cristae with loss of CJs than any single deletion indicating overlapping roles in formation of CJs. Using a combination of complexome profiling, STED nan  ...[more]

Similar Datasets

Proteomics Complexome profiling of MICOS complex, impact of subunits MIC26 and MIC27
2020-07-28 | PXD016732 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes from patients with mutation in NDUFC2 gene
2020-08-10 | PXD014936 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes from patients with mutation in NDUFAF8 gene
2020-03-05 | PXD015749 | Pride
Proteomics Complexome profiling of brain mitochondrial membranes from Clpp-/- mouse
2022-02-17 | PXD025478 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes to study the molecular consequences of DNAJC30 mutations in HEK cells
2021-09-09 | PXD022340 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes to study assembly of complex III and supercomplex stability in patients with defects in UQCRH gene
2021-09-20 | PXD022856 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes to study the molecular consequences of DNAJC30 mutations
2021-09-09 | PXD021385 | Pride
Proteomics Complexome profiling of mitochondrial protein complexes from patients with mutations in either DNAJC30 or ACAD9 compared to an age and gender matched control
2021-09-09 | PXD021386 | Pride
Proteomics Mitochondrial protein complexes from a patient with mutations in DNAJC30 compared to an age and gender matched healthy control
2021-09-09 | PXD021500 | Pride
Proteomics Dynamic complexome profiling identifies impaired turnover of specific complex I subunits in a patient with mutations in DNAJC30.
2021-09-09 | PXD021499 | Pride