Interactome of endogenously HA-tagged GABARAPL2
Ontology highlight
ABSTRACT: We selected GABARAPL2endoHA cells for a proof-of-principle immunoprecipitation (IP) followed by mass spectrometric (MS) analysis to identify new binding partner candidates of a hATG8 family member at endogenous levels. To distinguish between candidates that bind preferentially to PE-conjugated versus unconjugated GABARAPL2 we treated stable isotope labeling with amino acids in cell culture (SILAC)-labeled GABARAPL2endoHA cells with Torin1 and BafA1 (light) or ATG7 inhibitor (heavy).
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Hela Cell
DISEASE(S): Cervix Carcinoma
SUBMITTER: Christian Behrends
LAB HEAD: Christian Behrends
PROVIDER: PXD016734 | Pride | 2020-09-01
REPOSITORIES: Pride
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