Digestion of a variety of synthetic peptides by proteasomes

ABSTRACT: Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or recombine their fragments thereby generating novel sequences, i.e. spliced peptides via a process called proteasome-catalysed peptide splicing (PCPS). In order to gain more insight into these processes, we here perform in vitro digestions of 55 synthetic polypeptide substrates by proteasome isoforms followed by LC-MS/MS measurements using different Mass Spectrometers.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Artem Mansurkhodzhaev  

LAB HEAD: Juliane Liepe

PROVIDER: PXD016782 | Pride | 2020-04-14

REPOSITORIES: Pride