Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics

ABSTRACT: A differential lysis approach was developed with different strength of lysis buffers (urea and/or sodium dodecyl sulfate) and applied on human stool samples to separate different microbial species to increase the depth of metaproteomic measurements. We showed that the lysates dominating by proteins from Gram-positive (G+) or negative bacteria (G-) can be achieved by differential lysis

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

TISSUE(S): Colon

SUBMITTER: Jiaqin Wang  

LAB HEAD: Daniel Figeys

PROVIDER: PXD016787 | Pride | 2023-03-27

REPOSITORIES: Pride