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Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics

ABSTRACT: A differential lysis approach was developed with different strength of lysis buffers (urea and/or sodium dodecyl sulfate) and applied on human stool samples to separate different microbial species to increase the depth of metaproteomic measurements. We showed that the lysates dominating by proteins from Gram-positive (G+) or negative bacteria (G-) can be achieved by differential lysis

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

TISSUE(S): Colon

SUBMITTER: Jiaqin Wang

LAB HEAD: Daniel Figeys

PROVIDER: PXD016787 | Pride | 2023-03-27

REPOSITORIES: Pride

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Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics.

Wang Jiaqin J Zhang Xu X Li Leyuan L Ning Zhibin Z Mayne Janice J Schmitt-Ulms Cian C Walker Krystal K Cheng Kai K Figeys Daniel D

Analytical chemistry 20200309 7

Changes in microbiome composition and function have been linked to human health and diseases. Metaproteomics provides invaluable functional information on the state of a microbiome. However, lower-abundance bacteria in complex microbiomes are difficult to observe by metaproteomics. In this study, stepwise differential lysis protocols were developed for human stool microbiomes to separate different microbial species and to increase the depth of metaproteomic measurements. We achieved differential ...[more]

PMID: 32096399

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