Dataset Information

Proteomic analysis of Acinetobacter baylyi ADP1 under exposure of disinfectants

ABSTRACT: The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type Acinetobacter baylyi ADP1, and its protein response under the exposure of disinfectants, including chloramine and free chlorine. The concentrations of disinfectants were 10 mg/L. The group without dosing disinfectant was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of disinfectants on translational levels can be revealed.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Acinetobacter Sp.

SUBMITTER: Jianhua Guo

LAB HEAD: Jianhua Guo

PROVIDER: PXD016794 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress.

Zhang Shuai S Wang Yue Y Lu Ji J Yu Zhigang Z Song Hailiang H Bond Philip L PL Guo Jianhua J

The ISME journal 20210503 10

The bacterial infection that involves antimicrobial resistance is a rising global threat to public health. Chlorine-based water disinfection processes can inactivate antibiotic resistant bacteria. However, at the same time, these processes may cause the release of antibiotic resistance genes into the water as free DNA, and consequently increase the risk to disseminate antibiotic resistance via natural transformation. Presently, little is known about the contribution of residual chlorine affectin ...[more]

PMID: 33941886

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Project description:The aim of the research was to investigate the regulation of gene expression in ovine blood leukocytes during ACTH challenge and the effect of effects of dietary administration of botanicals to modulate the stress related response. Six homogeneous groups of Sarda sheep were used, one negative control (CT) and five injected with ACTH to mimic biological stress. Afterwards four of the five ACTH treated groups were separately and fed with extract from Echinacea angustifolia roots (PO), with extract from Echinacea angustifolia flowers (EA), with extracts from Andrographis paniculata (AP) and with Larix decidua sawdust (LB). Control groups (CT and ACTH) were fed with a basal diet. RNA was extracted from blood samples collected before (T0) and after 3 (T3) and 51 (T51) hours from the first ACTH injection and transcriptome analysis was performed using a custom oligoarray. The injection of ACTH caused a down regulation of transcripts (95.5%, 232 over 243; P<0.01) at T3, whilst the administration with the diet of AP, EA, LB and PO determined a predominant up regulation. At T51, the down regulated transcripts of the ACTH group in comparison to CT group were lower (36.2%, 142 over 392; P<0.01), whilst for the animals receiving plant extracts with the diet the percentage of down regulated transcripts increased, in particular for LB group (85.8 %). Biological pathways affected by the injection of the adrenocorticotrophic hormone include the MAPK signaling pathway, the p53 signaling pathway, the TGF-beta signaling pathway and the T-cell receptor signaling pathway. The most promising botanicals for the control of induced stress appeared Echinacea angustifolia and Larix decidua, in particular both showing a target for the NR4A1 gene, a glucocorticoid receptor involved in the cell trafficking after cortisol secretion. The RNA extracted from blood samples and depleted from globinic mRNA was used for the preparation of pools. Samples were pooled together within group (6 groups) and time of sampling (3 times), including in the pool an equal quantity of RNA for each sheep. Eighteen pools were obtained for the hybridizations, that were performed in duplicate (36 slides).

Dataset Information

Proteomic analysis of Acinetobacter baylyi ADP1 under exposure of disinfectants

Publications

Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress.

Similar Datasets

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