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PRG-1 Immunoprecipitation in C. Elegans

ABSTRACT: Co-Immunoprecipitation of PRG1 in Caenorhabditis elegans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Scott Greig

LAB HEAD: Angus Lamond

PROVIDER: PXD016838 | Pride | 2020-09-11

REPOSITORIES: Pride

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DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans.

Suen Kin Man KM Braukmann Fabian F Butler Richard R Bensaddek Dalila D Akay Alper A Lin Chi-Chuan CC Milonaitytė Dovilė D Doshi Neel N Sapetschnig Alexandra A Lamond Angus A Ladbury John Edward JE Miska Eric Alexander EA

Nature communications 20200825 1

Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but ...[more]

PMID: 32843637

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