Dataset Information

Effect of 1-methylcyclopropene (1-MCP) and ozone (Ο 3 ) post-harvest treatments on the proteomic profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage

ABSTRACT: Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O3) induced scald symptoms in ‘Granny Smith’ apple fruits during subsequent ripening at room temperature. In contrast, treatment with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) reversed this O3-induced scald effect. Amino acids, including branched-chain amino acids, were the most strongly induced metabolites in peel tissue of 1-MCP treated fruits. Proteins involved in oxidative stress and protein trafficking were differentially accumulated prior to and during scald development. Genes involved in photosynthesis, flavonoid biosynthesis and ethylene signaling displayed significant alterations in response to 1-MCP and O3. Analysis of regulatory module networks identified putative transcription factors (TFs) that could be involved in scald. Subsequently, a transcriptional network of the genes-proteins-metabolites and the connected TFs was constructed. This approach enabled identification of several genes co-regulated by TFs, notably encoding glutathione S-transferase (GST) protein(s) with distinct signatures following 1-MCP and O3 treatments. Overall, this study is an important contribution to future functional studies and breeding programs for this fruit, aiding to the development of improved apple cultivars to superficial scald.

OTHER RELATED OMICS DATASETS IN: GSE133456

INSTRUMENT(S):

ORGANISM(S): Malus Domestica

TISSUE(S): Plant Cell

SUBMITTER: Martina Samiotaki

LAB HEAD: Athanassios Molassiotis

PROVIDER: PXD016849 | Pride | 2020-05-27

REPOSITORIES: Pride

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Publications

Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald.

Karagiannis Evangelos E Tanou Georgia G Scossa Federico F Samiotaki Martina M Michailidis Michail M Manioudaki Maria M Laurens François F Job Dominique D Fernie Alisdair R AR Orsel Mathilde M Molassiotis Athanassios A

Frontiers in plant science 20200213

Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O<sub>3</sub>) induced scald symptoms in 'Granny Smith' apple fruits during subsequent ripening at room temper ...[more]

PMID: 32117359

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