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Comparative proteomic analysis revealed complex responses to Bordetella bronchiseptica infections in spleen in rabbits

ABSTRACT: Thirty five-week-old healthy Newzealand meet rabbits were used for bacteria infection. Bb infections were performed as the following: Finally, the dosage was 4.6×109CFU, and the ear vein was used in rabbits in the challenge group. Blood samples were collected for anticoagulant blood cell assay and serum isolation and preservation at 7 days after challenge. Six rabbits in the attack group died 10 days after the attack. All rabbits were euthanatized at the end of the experiment. Nine of the rabbits in each group were taken for spleen extraction.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Spleen

SUBMITTER: Chenwen Xiao

LAB HEAD: Xiao Chenwen

PROVIDER: PXD016866 | Pride | 2020-08-24

REPOSITORIES: Pride

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Comparative Proteomic Analysis Reveals Complex Responses to Bordetella bronchiseptica Infections in the Spleen of Rabbits.

Xiao Chenwen C Huang Yee Y Wei Qiang Q Liu Yan Y Ji Quanan Q Li Ke K Bao Guolian G

Proteomics 20201001 24

Bordetella bronchiseptica (B. bronchiseptica) causes a respiratory disease in rabbits. To determine the proteins of B. bronchiseptica in rabbits related to the disease, differentially accumulated proteins in B. bronchiseptica-infected cells are identified by comparative proteomic analysis. Comparative proteomic analysis detects 5814 proteins and quantifies 4854 of these. Fifty eight upregulated and 38 downregulated proteins are identified in spleen tissue after B. bronchiseptica infection of rab ...[more]

PMID: 32820866

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