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RNA binding proteins modification by drought stress

ABSTRACT: RNA-binding proteins are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via RNA-binding domains and these interactions affect RNA availability for translation, RNA stability and turn-over thus affecting both RNA and protein expression essential for developmental and stimulus specific responses. Here we investigate the effect of severe drought stress on the RNA-binding proteome to gain insights into the mechanisms that govern drought stress responses at the systems level

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: claudius Marondedze

LAB HEAD: Christoph Gehring

PROVIDER: PXD016883 | Pride | 2020-05-27

REPOSITORIES: Pride

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Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.

Marondedze Claudius C Thomas Ludivine L Lilley Kathryn S KS Gehring Chris C

Frontiers in molecular biosciences 20200121

The spliceosome processes RNAs from a pre-RNA state to a mature mRNA thereby influencing RNA availability for translation, localization, and turnover. It consists of complex structures containing RNA-binding proteins (RBPs) essential for post-transcriptional gene expression control. Here we investigate the dynamic modifications of spliceosomal RBPs under stress and in particular drought stress. We do so by mRNA interactome capture in <i>Arabidopsis thaliana</i> using label free quantitation. Thi ...[more]

PMID: 32039234

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