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Peptide mapping of SRSF7_RRM isoforms in murine P19 cells

ABSTRACT: Here we mapped peptides of mouse SRSF7_RRM purified from P19 cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Michaela Müller-McNicoll

PROVIDER: PXD016884 | Pride | 2020-05-27

REPOSITORIES: Pride

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SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly.

Königs Vanessa V de Oliveira Freitas Machado Camila C Arnold Benjamin B Blümel Nicole N Solovyeva Anfisa A Löbbert Sinah S Schafranek Michal M Ruiz De Los Mozos Igor I Wittig Ilka I McNicoll Francois F Schulz Marcel H MH Müller-McNicoll Michaela M

Nature structural & molecular biology 20200302 3

SRSF7 is an essential RNA-binding protein whose misexpression promotes cancer. Here, we describe how SRSF7 maintains its protein homeostasis in murine P19 cells using an intricate negative feedback mechanism. SRSF7 binding to its premessenger RNA promotes inclusion of a poison cassette exon and transcript degradation via nonsense-mediated decay (NMD). However, elevated SRSF7 levels inhibit NMD and promote translation of two protein halves, termed Split-ORFs, from the bicistronic SRSF7-PCE transc ...[more]

PMID: 32123389

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