Dataset Information

Proteomic analysis of the proteins secreted by the infection cushions of Botrytis cinerea.

ABSTRACT: Gray mold disease affects fruits, vegetables and ornemental plants around the world, causing considerable losses every year. Its causing agent, the fungus Botrytis cinerea, produces infection cushions (IC) that are complex appressorial structure dedicated to the penetration of the plant tissues. A microarray analysis identified 1,231 up-regulated genes in IC-enriched mycelium, among which several genes coding for putative secreted proteins. These data were supported by qPCR analysis in vitro and in planta. They highlight a secretion of proteins involved in virulence like proteases, plant cell wall degrading enzymes and necrosis inducers. To validate these transcriptomic data, a proteomic analysis of the IC exo-proteome is required.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Dupuy Jean-William

LAB HEAD: Nathalie Poussereau

PROVIDER: PXD016885 | Pride | 2021-06-10

REPOSITORIES: Pride

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Publications

The infection cushion of Botrytis cinerea: a fungal 'weapon' of plant-biomass destruction.

Choquer Mathias M Rascle Christine C Gonçalves Isabelle R IR de Vallée Amélie A Ribot Cécile C Loisel Elise E Smilevski Pavlé P Ferria Jordan J Savadogo Mahamadi M Souibgui Eytham E Gagey Marie-Josèphe MJ Dupuy Jean-William JW Rollins Jeffrey A JA Marcato Riccardo R Noûs Camille C Bruel Christophe C Poussereau Nathalie N

Environmental microbiology 20210222 4

The necrotrophic plant-pathogen fungus Botrytis cinerea produces multicellular appressoria dedicated to plant penetration, named infection cushions (IC). A microarray analysis was performed to identify genes upregulated in mature IC. The expression data were validated by RT-qPCR analysis performed in vitro and in planta, proteomic analysis of the IC secretome and biochemical assays. 1231 upregulated genes and 79 up-accumulated proteins were identified. The data support the secretion of effectors ...[more]

PMID: 33538395

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Project description:Botrytis cinerea (gray mold) is one of the most destructive pathogens of cherry tomatoes, causing fruit decay and economic loss. Fludioxonil is an effective fungicide widely used for crop protec-tion and is essential for controlling tomato gray mold. The emergence of fungicide-resistant strains has made the control of Botrytis cinerea more difficult. While the genome of Botrytis cinerea is available, there are few reports regarding the large-scale functional annotation of the genome using expressed genes derived from transcriptomes, and the mechanism(s) underlying such flu-dioxonil resistance remain unclear. The present study prepared RNA-sequencing (RNA-seq) li-braries for three Botrytis cinerea strains [two highly resistant (LR and FR) versus one highly sen-sitive (S) to fludioxonil], with and without fludioxonil treatment, to identify fludioxonil responsive genes that facilitate fungicide resistance. Functional enrichment analysis identified nine resistant related DEGs in the fludioxonil-induced LR and FR transcriptome that were simultaneously up regulated, and seven resistant related DEGs down regulated. These included adenosine tri-phosphate (ATP)-binding cassette (ABC) transporter-encoding genes, major facilitator super-family (MFS) transporter-encoding genes, and the high-osmolarity glycerol (HOG) pathway homologues or related genes. The expression patterns of twelve out of the sixteen fludioxo-nil-responsive genes, obtained from the RNA-sequence data sets were validated using quantita-tive real-time PCR (qRT-PCR). Based on RNA-sequence analysis it was found that fugal HHKs, like BOS1, BcHHK2, and Bchhk17, were in some way involved in the fludioxonil resistance of B. cinerea, in addition, a number of ABC and MFS transporter genes that were not reported before, such as BcATRO, BMR1, BMR3, BcNMT1, BcAMF1, BcTOP1, BcVBA2, and BcYHK8 were differen-tially expressed in the fludioxonil-resistant strains, indicating that overexpression of these efflux transporters located in the plasma membranes played a crucial role in the fludioxonil resistant mechanism of B. cinerea. These lines of evidence together allowed us to draw a general portrait of the anti-fludioxonil mechanisms for Botrytis cinerea, and the assembled and annotated transcrip-tome data provide valuable genomic resources for further study of the molecular mechanisms of B. cinerea resistance to fludioxonil.

Dataset Information

Proteomic analysis of the proteins secreted by the infection cushions of Botrytis cinerea.

Publications

The infection cushion of Botrytis cinerea: a fungal 'weapon' of plant-biomass destruction.

Similar Datasets

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