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Phosphorylation regulates secretion of Mycobacterium tuberculosisvirulence mediators ESAT6 and CFP10

ABSTRACT: Virulence effectors secreted by Mycobacterium tuberculosis help subvert host immune mechanisms and therefore are critical for establishment of infection and pathogenesis. However, knowledge in terms of signaling mechanisms thatmodulate the secretion of virulence factors is sparse. Here using high-throughput mass-spectrometric analysis of mycobacterial secretome,phospho-proteome and phospho-secretome combined with empirical validations, we show an unprecedented regulation of mycobacterial secretion via protein phosphorylation. The intricate relationship between phosphorylation and secretion was revealed by system-level analysis of PPI network superimposed with secretome and phospho-proteome profiles. This study establishes phosphorylation as a keydeterminant of secretion of virulence factors and unveils as yet unexplored targets for therapeutic interventions.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Bacteria Mycobacterium Tuberculosis H37rv

DISEASE(S): Tuberculosis

SUBMITTER: basanti malakar

LAB HEAD: Vinay Kumar Nandicoori

PROVIDER: PXD017004 | Pride | 2023-09-18

REPOSITORIES: Pride

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