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Crosslinking mass spectrometry (CLMS) analysis of monoubiquitinated FANCD2-FANCI complex

ABSTRACT: Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion. In this study, monoubiquitinated FANCD2-FANCI complex was characterized using crosslinking mass spectrometry in order to provide in sights into the 3D structure of the complex.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Zhuo Chen

LAB HEAD: Juri Rappsilber

PROVIDER: PXD017020 | Pride | 2020-03-09

REPOSITORIES: Pride

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FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair.

Alcón Pablo P Shakeel Shabih S Chen Zhuo A ZA Rappsilber Juri J Patel Ketan J KJ Passmore Lori A LA

Nature structural & molecular biology 20200217 3

Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion. Here, we use cryo-EM to determine the structures of recombinant chicken FANCD2 and FANCI complexes. FANCD2-FANCI adopts a closed conforma ...[more]

PMID: 32066963

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