Crosslinking mass spectrometry (CLMS) analysis of monoubiquitinated FANCD2-FANCI complex

ABSTRACT: Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion. In this study, monoubiquitinated FANCD2-FANCI complex was characterized using crosslinking mass spectrometry in order to provide in sights into the 3D structure of the complex.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Zhuo Chen  

LAB HEAD: Juri Rappsilber

PROVIDER: PXD017020 | Pride | 2020-03-09

REPOSITORIES: Pride