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Cullin 3 Mouse Brain TMT-based Proteomics Analysis

ABSTRACT: This project consists in 3 datasets: - Samples from adult mouse brains (Hippocampus, Cortex and Cerebellum): Cul3 +/- or +/+; 5 animals per condition. - Samples from embryonic mouse cortex: either Cul3 +/- or +/+ - Samples from embryonic mouse cortex: Cul3 fl/fl Emx-1-Cre ("hom"); Cul3 +/fl Emx1-Cre ("het") or Cul3+/fl ("control")

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Autism Spectrum Disorder

SUBMITTER: Armel Nicolas

LAB HEAD: Gaia Novarino

PROVIDER: PXD017040 | Pride | 2021-03-26

REPOSITORIES: Pride

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Publications

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Morandell Jasmin J Schwarz Lena A LA Basilico Bernadette B Tasciyan Saren S Dimchev Georgi G Nicolas Armel A Sommer Christoph C Kreuzinger Caroline C Dotter Christoph P CP Knaus Lisa S LS Dobler Zoe Z Cacci Emanuele E Schur Florian K M FKM Danzl Johann G JG Novarino Gaia G

Nature communications 20210524 1

De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 (CUL3) lead to autism spectrum disorder (ASD). In mouse, constitutive Cul3 haploinsufficiency leads to motor coordination deficits as well as ASD-relevant social and cognitive impairments. However, induction of Cul3 haploinsufficiency later in life does not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during a critical developmental window. Here we show that Cul3 is essential to regulate neu ...[more]

PMID: 34031387

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