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Excess copper-induced alterations of protein profiles by a 2-DE based MS method and related physiological parameters in Citrus leaves

ABSTRACT: After long-term Copper-toxicity treatment,2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis and Citrus grandis leaves samples.

INSTRUMENT(S): TripleTOF 5600, MALDI Synapt MS

ORGANISM(S): Citrus Sinensis X Citrus Reticulata

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lin-Tong Yang

LAB HEAD: Lin-Tong Yang

PROVIDER: PXD017049 | Pride | 2020-05-27

REPOSITORIES: Pride

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Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves.

Huang Wei-Lin WL Wu Feng-Lin FL Huang Hui-Yu HY Huang Wei-Tao WT Deng Chong-Ling CL Yang Lin-Tong LT Huang Zeng-Rong ZR Chen Li-Song LS

Plants (Basel, Switzerland) 20200228 3

This present study examined excess copper (Cu) effects on seedling growth, leaf Cu concentration, gas exchange, and protein profiles identified by a two-dimensional electrophoresis (2-DE) based mass spectrometry (MS) approach after Citrus sinensis and Citrus grandis seedlings were treated for six months with 0.5 (control), 200, 300, or 400 μM CuCl2. Forty-one and 37 differentially abundant protein (DAP) spots were identified in Cu-treated C. grand ...[more]

PMID: 32121140

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2016-08-24 | E-GEOD-85958 | biostudies-arrayexpress

Root adaptive responses to aluminum-toxicity revealed by RNA-Seq in two citrus species with contrasting aluminum tolerance

Project description:Background: Limited data are available on aluminum (Al)-toxicity-induced alterations of gene profiles in woody plants. Seedlings of Al-tolerant Citrus sinensis and Al-intolerant Citrus grandis were fertigated with nutrient solution containing 0 and 1.0 mM AlCl3•6H2O. Thereafter, we investigated the Al-toxicity-induced alterations of transcriptomics in roots by RNA-Seq. Results: Using RNA-seq, we isolated 1293 (990) up- and 1377 (915) downregulated genes from Al-treated C. grandis (C. sinensis) roots. Clearly, gene expression was less affected by Al-toxicity in C. sinensis roots than in C. grandis ones. Several Al-toxicity-responsive genes homologous to known Al-tolerance genes: Al-activated malate transporter, multidrug and toxic compound extrusion (MATE), IRON REGULATED/ferroportin 1, sensitive to proton rhizotoxicity 1 and monogalactosyldiacylglycerol synthase were identified in citrus roots. However, Al-induced upregulation of all these genes was stronger in C. grandis roots than in C. sinensis ones except for MATEs. Genes related to signal transduction, and sulfur transport and metabolism might also play a role in the higher Al-tolerance of C. sinensis. Conclusions: This is the first comparative investigation of transcriptomic responses in Al-treated citrus roots. There were common and unique mechanisms for citrus Al-tolerance. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance.

2016-08-24 | GSE85958 | GEO

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2018-01-08 | GSE108837 | GEO

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