Proteomics

Dataset Information

2

INTEGRATED MULTI-OMICS ANALYSES OF MAIZE REVEAL ROLES FOR AUTOPHAGIC DURING FIXED CARBON STARVATION


ABSTRACT: The turnover of cytoplasmic material via autophagic encapsulation and delivery to vacuoles is essential for recycling cellular constituents, especially under nutrient-limiting conditions. To determine how cells/tissues rely on autophagy, we applied in-depth multi-omic analyses to study maize (Zea mays) autophagy mutants grown after fixed carbon starvation. Surprisingly, broad alterations in the leaf metabolome were evident in plants missing the core autophagy component ATG12 even without stress, particularly affecting products of lipid turnover and secondary metabolites, which were underpinned by substantial changes in the transcriptome and/or proteome. Cross-comparison of mRNA and protein abundances allowed for the identification of organelles, protein complexes, and individual proteins targeted for selective autophagic clearance, and revealed several processes controlled by this catabolism. Fixed carbon starvation induced the proteins involved in alternative respiration together with the upregulation of various alternative catabolic processes. Collectively, we describe a facile proteomic strategy to survey autophagic substrates, and show that autophagy has a greater than expected influence in sculpting plant proteomes and membranes both before and during nitrogen stress.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zea mays  

TISSUE(S): Leaf

DISEASE(S): Not Available

SUBMITTER: Fionn McLoughlin  

LAB HEAD: Richard D Vierstra

PROVIDER: PXD017077 | Pride | 2020-07-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
3rdleaf-Cv3.msf Msf
3rdleaf-Cv3.pep.xml Pepxml
W22+CRep1_T1.raw Raw
W22+CRep1_T2.raw Raw
W22+CRep1_T3.raw Raw
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Publications

Autophagy Plays Prominent Roles in Amino Acid, Nucleotide, and Carbohydrate Metabolism during Fixed-Carbon Starvation in Maize.

McLoughlin Fionn F   Marshall Richard S RS   Ding Xinxin X   Chatt Elizabeth C EC   Kirkpatrick Liam D LD   Augustine Robert C RC   Li Faqiang F   Otegui Marisa S MS   Vierstra Richard D RD  

The Plant cell 20200702 9


Autophagic recycling of proteins, lipids, nucleic acids, carbohydrates, and organelles is essential for cellular homeostasis and optimal health, especially under nutrient-limiting conditions. To better understand how this turnover affects plant growth, development, and survival upon nutrient stress, we applied an integrated multiomics approach to study maize (<i>Zea mays</i>) autophagy mutants subjected to fixed-carbon starvation induced by darkness. Broad metabolic alterations were evident in l  ...[more]

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