Dataset Information

Staphylococcus saprophyticus proteomic analyses elucidate differences in the protein repertories among clinical strains related to virulence and persistence

ABSTRACT: In our work, we used a proteomic approach to compare three S. saprophyticus strains possessing different patterns of capsule: the ATCC 15305 possessing capsule, the non-capsular 7108 strain and the highly capsular strain 9325.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Staphylococcus Saprophyticus

SUBMITTER: CLAYTON BORGES

LAB HEAD: CLAYTON LUIZ BORGES

PROVIDER: PXD017112 | Pride | 2020-01-29

REPOSITORIES: Pride

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Publications

Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence.

Silva Karla Christina Sousa KCS Silva Lana O'Hara Souza LOS Silva Guilherme Algusto Alves GAA Borges Clayton Luiz CL Novaes Evandro E Paccez Juliano Domiraci JD Fontes Wagner W Giambiagi-deMarval Marcia M Soares Célia Maria de Almeida CMA Parente-Rocha Juliana Alves JA

Pathogens (Basel, Switzerland) 20200119 1

Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was p ...[more]

PMID: 31963821

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Project description:Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.

Dataset Information

Staphylococcus saprophyticus proteomic analyses elucidate differences in the protein repertories among clinical strains related to virulence and persistence

Publications

<i>Staphylococcus saprophyticus</i> Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence.

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