Comprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases

ABSTRACT: We analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acquisition) on Sciex TripleTof 5600+ mass‐spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano‐HPLC system (Eksigent). Dataset covers 16 samples.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Olga Bukato  

LAB HEAD: Petr Sergiev

PROVIDER: PXD017171 | Pride | 2020-01-21

REPOSITORIES: Pride