Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Brain, Epithelial Cell, Colon, Oocyte
DISEASE(S): Colon Cancer,Glioblastoma,Malignant Neoplasm Of Ovary
SUBMITTER: Fabian Coscia
LAB HEAD: Matthias Mann
PROVIDER: PXD017269 | Pride | 2020-04-02
REPOSITORIES: Pride
Coscia Fabian F Doll Sophia S Bech Jacob Mathias JM Schweizer Lisa L Mund Andreas A Lengyel Ernst E Lindebjerg Jan J Madsen Gunvor Iben GI Moreira José Ma JM Mann Matthias M
The Journal of pathology 20200501 1
Formalin fixation and paraffin-embedding (FFPE) is the most common method to preserve human tissue for clinical diagnosis, and FFPE archives represent an invaluable resource for biomedical research. Proteins in FFPE material are stable over decades but their efficient extraction and streamlined analysis by mass spectrometry (MS)-based proteomics has so far proven challenging. Herein we describe a MS-based proteomic workflow for quantitative profiling of large FFPE tissue cohorts directly from hi ...[more]