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The porcine granulocyte proteome: expression changes in a transgenic pig model for diabetes

ABSTRACT: As transgenic INSC94Y-pigs develop a stable diabetic phenotype we were able to study the influence of elevated blood glucose levels on primary immune cells in vivo. We investigated the neutrophil proteome and compared data of transgenic animals to a control group. A total of 2371 proteins describing the whole neutrophil proteome were identified, including 396 proteins (17% of proteome) newly associated to expression in porcine neutrophils. Our studies provide novel information on the porcine granulocyte proteome and contribute to a better understanding of molecular mechanisms involved in altered immune cell function in diabetes. The data presented here is highly relevant for veterinary medicine and has translational quality for diabetes in human.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Neutrophil, Blood

DISEASE(S): Permanent Neonatal Diabetes Mellitus

SUBMITTER: Roxane Degroote

LAB HEAD: Cornelia Annette Deeg

PROVIDER: PXD017274 | Pride | 2020-06-04

REPOSITORIES: Pride

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Proteome profile of neutrophils from a transgenic diabetic pig model shows distinct changes.

Weigand Maria M Degroote Roxane L RL Amann Barbara B Renner Simone S Wolf Eckhard E Hauck Stefanie M SM Deeg Cornelia A CA

Journal of proteomics 20200527

INS<sup>C94Y</sup> transgenic pigs develop a stable diabetic phenotype early after birth and therefore allow studying the influence of hyperglycemia on primary immune cells in an early stage of diabetes mellitus in vivo. Since immune response is altered in diabetes mellitus, with deviant neutrophil function discussed as one of the possible causes in humans and mouse models, we investigated these immune cells in INS<sup>C94Y</sup> transgenic pigs and wild type controls at protein level. A total o ...[more]

PMID: 32470542

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