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Mouse germ cell proteome from spermatogonia to round spermatids analysis by LC-MSMS

ABSTRACT: The data contains 135 raw data produced from mass spectrometry (QExactive HF, Thermo Scientific), the bio-samples were sperm cells collected at nine sub-stages of mouse spermatogenesis. Proteins from each bio-samples were extracted and digested to peptides, then separated to five fractions and triplicated analyzed by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Qidan Li

LAB HEAD: Siqi Liu

PROVIDER: PXD017284 | Pride | 2020-12-01

REPOSITORIES: Pride

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Prediction and Validation of Mouse Meiosis-Essential Genes Based on Spermatogenesis Proteome Dynamics.

Fang Kailun K Li Qidan Q Wei Yu Y Zhou Changyang C Guo Wenhui W Shen Jiaqi J Wu Ruoxi R Ying Wenqin W Yu Lu L Zi Jin J Zhang Yuxing Y Yang Hui H Liu Siqi S Chen Charlie Degui CD

Molecular & cellular proteomics : MCP 20210106

The molecular mechanism associated with mammalian meiosis has yet to be fully explored, and one of the main reasons for this lack of exploration is that some meiosis-essential genes are still unknown. The profiling of gene expression during spermatogenesis has been performed in previous studies, yet few studies have aimed to find new functional genes. Since there is a huge gap between the number of genes that are able to be quantified and the number of genes that can be characterized by phenotyp ...[more]

PMID: 33257503

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