Dataset Information

Eudiplozoon nipponicum secretome analysis

ABSTRACT: Ectoparasitic flatworms from the family Diplozoidae (Platyhelminthes: Monogenea) represent serious hematophagous fish pathogens. Information related to the biochemical and molecular nature of the physiological processes is rather sporadic, as well as the knowledge of the molecules produced by monogeneans and their role in host-parasite interaction. Therefore, we performed a complex secretomic analysis of monogenean representative Eudiplozoon nipponicum for the purpose to identify functionally important protein molecules involved in these host-parasite interactions.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Eudiplozoon Nipponicum

SUBMITTER: Pavel Roudnický

LAB HEAD: Zbyněk Zdráhal

PROVIDER: PXD017293 | Pride | 2021-04-17

REPOSITORIES: Pride

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Publications

Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.

Vorel Jiří J Cwiklinski Krystyna K Roudnický Pavel P Ilgová Jana J Jedličková Lucie L Dalton John P JP Mikeš Libor L Gelnar Milan M Kašný Martin M

BMC genomics 20210415 1

<h4>Background</h4>Ectoparasites from the family Diplozoidae (Platyhelminthes, Monogenea) belong to obligate haematophagous helminths of cyprinid fish. Current knowledge of these worms is for the most part limited to their morphological, phylogenetic, and population features. Information concerning the biochemical and molecular nature of physiological processes involved in host-parasite interaction, such as evasion of the immune system and its regulation, digestion of macromolecules, suppression ...[more]

PMID: 33858339

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Project description:Filarial nematodes (superfamily Filarioidea) are responsible for an annual global health burden of approximately 6.3 million disability-adjusted life-years, which represents the greatest single component of morbidity attributable to helminths affecting humans. No vaccine exists for the major filarial diseases, lymphatic filariasis and onchocerciasis; in part because research on protective immunity against filariae has been constrained because the human-parasitic species cannot complete their lifecycles in laboratory mice. However, the rodent filaria Litomosoides sigmodontis has become a popular experimental model, as BALB/c mice are fully permissive for its development and reproduction. Here, we provide a comprehensive analysis of excretory-secretory products from L. sigmodontis across five lifecycle stages. Applying intensity-based quantification, we determined the abundance of 302 unique excretory-secretory proteins, of which 64.6% were present in quantifiable amounts only from gravid adult female nematodes. This lifecycle stage, together with immature first-stage larvae (microfilariae), released four proteins that have not previously been evaluated as vaccine candidates: a predicted 28.5 kDa filaria-specific protein, a zonadhesin and SCO-spondin-like protein, a vitellogenin, and a protein containing six metridin-like ShK toxin domains. Female nematodes also released two proteins derived from the obligate Wolbachia symbiont. Notably, excretory-secretory products from all parasite stages contained several uncharacterised members of the transthyretin-like protein family. Furthermore, biotin labelling revealed that redox proteins and enzymes involved in purinergic signalling were enriched on the adult nematode cuticle. Comparison of the L. sigmodontis adult secretome with that of the human–infective filarial nematode Brugia malayi (reported previously in three independent published studies) identified differences that suggest a considerable underlying diversity of potential immunomodulators. The molecules identified in L. sigmodontis excretory-secretory products show promise not only for vaccination against filarial infections, but for the amelioration of allergy and autoimmune diseases.

Dataset Information

Eudiplozoon nipponicum secretome analysis

Publications

Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.

Similar Datasets

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