Proteomics

Dataset Information

0

GFP-CSB and GFP-UVSSA Domain-specific interactors

ABSTRACT: The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA repair complex, including transcription factor IIH (TFIIH), is recruited are largely unknown. Here, we define the assembly mechanism of the TCR complex in human isogenic knockout cells. We show that TCR is initiated by RNAPIIobound CSB, which recruits CSA through a newly identified CSA-interaction motif (CIM). Once recruited, CSA facilitates the association of UVSSA with stalled RNAPIIo. Importantly, we find that UVSSA is the key factor that recruits the TFIIH complex in a manner that is stimulated by CSB and CSA. Together these findings reveal a sequential and highly cooperative assembly mechanism of TCR proteins and reveal the mechanism for TFIIH recruitment to DNA damage-stalled RNAPIIo to initiate repair.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Román González-Prieto  

LAB HEAD: Alfred C.O. Vertegaal

PROVIDER: PXD017329 | Pride | 2020-04-14

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA repair complex, including transcription factor IIH (TFIIH), is recruited are largely unknown. Here, we define the assembly mechanism of the TCR complex in human isogenic knockout cells. We show that TCR is initiated by RNAPIIo-bound CSB, which  ...[more]

Similar Datasets

Proteomics UV-specific and -indepent interactors
2020-04-14 | PXD013572 | Pride
Proteomics Structural basis of human transcription-DNA repair coupling
2022-02-17 | PXD025328 | Pride
Unknown Drosophila, which Lacks Canonical Transcription-Coupled Repair Proteins, Performs Transcription-Coupled Repair
2019-10-16 | GSE138846 | GEO
Proteomics ELOF1 anchors CRL4CSA to RNA pol II to activate transcription-coupled repair
2023-11-24 | PXD042388 | Pride
Unknown eXcision Repair-sequencing (XR-seq) to map UV induced damage in U2OS cells and in U2OS cells in which CSA or UVSSA genes were knocked out
2020-04-13 | GSE132840 | GEO
Proteomics Transcription-coupled DNA-Protein Crosslink repair by CSB and CRL4(CSA)-mediated degradation
2024-02-09 | PXD041679 | Pride
Unknown Gene expression regulated by CSA and CSB in neuroblastoma cell line
2019-03-29 | GSE129002 | GEO
Unknown Convergence of Cockayne Syndrome Group A and B Proteins at rRNA Transcription through Nucleolin Regulation
2020-03-16 | GSE122736 | GEO
Unknown Cockayne syndrome A and B proteins regulate the transcription arrest upon genotoxic stress through a ubiquitin/proteasome degradation process (ChIP-seq)
2017-12-01 | GSE87562 | GEO
Unknown Cockayne syndrome A and B proteins regulate the transcription arrest upon genotoxic stress through a ubiquitin/proteasome degradation process (RNA-seq)
2017-12-01 | GSE87540 | GEO