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CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion

ABSTRACT: The centriole-to-centrosome conversion is a mechanism of the centrosome maturation process that depends on the formation of a proper centriole wall structure. Beside the centriole wall biogenesis factors TUBD1 and TUBE1, CEP44 is involved in this process. Lack of CEP44 generates defects in the centriole wall structure and thus, it interferes with the centrosome maturation. POC1B was found to be an interactor of CEP44 in the immunoprecipitation and LC-MS experiment. The interaction and the function of this hit was validated with further independent biological experiments.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: elmar schiebel

LAB HEAD: Elmar Johannes Schiebel

PROVIDER: PXD017332 | Pride | 2020-03-24

REPOSITORIES: Pride

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CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion.

Atorino Enrico S ES Hata Shoji S Funaya Charlotta C Neuner Annett A Schiebel Elmar E

Nature communications 20200214 1

Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote c ...[more]

PMID: 32060285

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