Dataset Information

Identification of the initial molecular changes in response to Circulating Angiogenic Cells-mediated therapy in Critical Limb Ischemia

ABSTRACT: Circulating angiogenic cells (CACs) constitute promising candidates for cell therapy in critical limb ischemia (CLI) due to their assigned vascular regenerative properties. A label free MS-based quantitative approach was performed to identify protein changes related. We analyzed the initial molecular mechanisms triggered by human CACs after being administered to a murine model of CLI, in order to understand how these cells promote angiogenesis within the ischemic tissue.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Leg Muscle

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Lucía Beltrán-Camacho

LAB HEAD: Mª Carmen Duran-Ruiz

PROVIDER: PXD017661 | Pride | 2020-05-27

REPOSITORIES: Pride

ACCESS DATA

Publications

Identification of the initial molecular changes in response to circulating angiogenic cells-mediated therapy in critical limb ischemia.

Beltran-Camacho Lucia L Jimenez-Palomares Margarita M Rojas-Torres Marta M Sanchez-Gomar Ismael I Rosal-Vela Antonio A Eslava-Alcon Sara S Perez-Segura Mª Carmen MC Serrano Ana A Antequera-González Borja B Alonso-Piñero Jose Angel JA González-Rovira Almudena A Extremera-García Mª Jesús MJ Rodriguez-Piñero Manuel M Moreno-Luna Rafael R Larsen Martin Røssel MR Durán-Ruiz Mª Carmen MC

Stem cell research & therapy 20200306 1

<h4>Background</h4>Critical limb ischemia (CLI) constitutes the most aggressive form of peripheral arterial occlusive disease, characterized by the blockade of arteries supplying blood to the lower extremities, significantly diminishing oxygen and nutrient supply. CLI patients usually undergo amputation of fingers, feet, or extremities, with a high risk of mortality due to associated comorbidities. Circulating angiogenic cells (CACs), also known as early endothelial progenitor cells, constitute ...[more]

PMID: 32143690

Similar Datasets

Project description:Detailed information about stage-specific changes in gene expression is crucial for understanding the gene regulatory networks underlying development and the various signal transduction pathways contributing to morphogenesis. Here, we describe the global gene expression dynamics during early murine limb development, when cartilage, tendons, muscle, joints, vasculature, and nerves are specified and the musculoskeletal system of the limbs is established. We used whole-genome microarrays to identify genes with differential expression at 5 stages of limb development (E9.5 to 13.5), during fore-limb and hind-limb patterning. We found that the onset of limb formation is characterized by an up-regulation of transcription factors, which is followed by a massive activation of genes during E10.5 and E11.5 which tampers off at later time points. Among 3520 genes identified as significantly up-regulated in the limb, we find ~30% to be novel, dramatically expanding the repertoire of candidate genes likely to function in the limb. Hierarchical and stage-specific clustering identified expression profiles that correlate with functional programs during limb development and are likely to provide new insights into specific tissue patterning processes. Here we provide for the first time, a comprehensve analysis of developmentally regulated genes during murine limb development, and provide some novel insights into the expression dynamics governing limb morphogenesis. Fifty- one arrays were analyzed, consisting of whole fore-limb and hind-limb bud RNA (experimental) and whole embryo RNA (reference) samples from E9.5 to E13.5 DPC mouse (FVB strain). Embryos were not pooled to generate samples. Each time point has 3 to 5 biological replicates for limb bud samples, duplicates for whole embryos. Comparisons were made between limb bud samples and whole embryo at the same stage, fore-limb samples of different stages, hind-limb samples of different stages, and fore-limb samples compared to hind-limb samples at the same or the next stage.

Dataset Information

Identification of the initial molecular changes in response to Circulating Angiogenic Cells-mediated therapy in Critical Limb Ischemia

Publications

Identification of the initial molecular changes in response to circulating angiogenic cells-mediated therapy in critical limb ischemia.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure