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Analysis of IgG1 producing CHO cells

ABSTRACT: The effects of changes in bioprocessing parameters (temp, pH, DO) on anti-IL8 IgG1 producing Chinese hamster ovary (CHO) cells were studied on the level of host cell proteome expression using TMT-SPS-MS3.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Culture

SUBMITTER: Lisa Strasser

LAB HEAD: Jonathan Bones

PROVIDER: PXD017779 | Pride | 2021-04-09

REPOSITORIES: Pride

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Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS<sup>3</sup>: The Effects of Bioprocessing Conditions on Productivity and Product Quality.

Strasser Lisa L Farrell Amy A Ho Jenny T C JTC Scheffler Kai K Cook Ken K Pankert Patrick P Mowlds Peter P Viner Rosa R Karger Barry L BL Bones Jonathan J

Frontiers in bioengineering and biotechnology 20210409

The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in combination with optimization of various process parameters results in increasing product titers. To enable further improvements in manufacturing processes, detailed information about how certain parameters affect cellular mechanisms in the production cells, and thereby also the expressed drug substance, is required. Theref ...[more]

PMID: 33898396

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