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Proteomic characterization of the culture supernatant of Mycobacterium bovis in different growth stages

ABSTRACT: The goal of this study is to identify the different proteins secreted by Mycobacterium bovis in the culture media at different stages of bacterial growth. A field strain of M. bovis was allowed to grow in a culture media and culture supernatant was collected at three-time points representing approximately three different phases of growth. The supernatant was digested by trypsin and analyzed by LC-MS/MS analysis

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mycobacterium Bovis Bacteria

TISSUE(S): Cell Culture

SUBMITTER: Nadia Assal

LAB HEAD: Min Lin

PROVIDER: PXD017817 | Pride | 2021-12-30

REPOSITORIES: Pride

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Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages.

Assal Nadia N Rennie Bryan B Walrond Lisa L Cyr Terry T Rohonczy Liz L Lin Min M

Biochemistry and biophysics reports 20211023

This study aimed to identify proteins secreted by Mycobacterium bovis into culture medium at different stages of bacterial growth. A field strain of M. bovis was grown in Middlebrook 7H9 media and culture supernatant was collected at three-time points representing three different phases of growth (early exponential, late exponential, and stationary phases). Supernatants were double filtered, digested by trypsin and analyzed by LC-MS/MS. The study found 15, 21, and 16 proteins in ea ...[more]

PMID: 34746449

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