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HEK293 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)

ABSTRACT: HEK293 cells with two genotype (WT or ATCG5 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Alban Ordureau

LAB HEAD: Wade Harper

PROVIDER: PXD017852 | Pride | 2020-07-12

REPOSITORIES: Pride

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Systematic quantitative analysis of ribosome inventory during nutrient stress.

An Heeseon H Ordureau Alban A Körner Maria M Paulo Joao A JA Harper J Wade JW

Nature 20200701 7815

Mammalian cells reorganize their proteomes in response to nutrient stress through translational suppression and degradative mechanisms using the proteasome and autophagy systems1,2. Ribosomes are central targets of this response, as they are responsible for translation and subject to lysosomal turnover during nutrient stress3-5. The abundance of ribosomal (r)-proteins (around 6% of the proteome; 107 copies per cell)6,7 and their high arginine and lysin ...[more]

PMID: 32612236

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HCT116 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)

Project description:HCT116 cells with various genotype (WT, ATCG5 KO, RB1CC1 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex single shot.

2020-07-13 | PXD017853 | Pride

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HEK293T cell degradome (pulse chase) analysis upon 12h Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)

Project description:HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 12h. Samples are Biotin-click, and streptavidin enriched.

2020-04-30 | PXD017857 | Pride

HEK293T cell proteome remodeling upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)

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Project description:HEK293T subject to Torin1 treatment or amino acid withdraw dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMT 10plex labelling was used. Three genotypes, WT (24 fractions), ATG7 KO (HA-9-53-1 single shot), RB1CC1 KO (HA-9-53-2 single shot).

2020-04-30 | PXD018252 | Pride

HEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 6 from An et al (2020)

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2020-04-30 | PXD017856 | Pride

HEK293T cell nuclear & cytosolic phospho-proteome remodelling upon amino acid withdrawal. Related to Supplementary Table 4 from An et al (2020)

Project description:HEK293T Phospho proteome cytosolic vs nuclear fractionation upon short (3h) amino acids withdrawal.

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Mammalian Cell Proliferation Requires Noncatalytic Functions of O-GlcNAc Transferase

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2021-09-09 | PXD022654 | Pride

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2019-06-28 | PXD013922 | Pride

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