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Tauroursodeoxycholic acid-induced neural stem cell proliferation

ABSTRACT: Tauroursodeoxycholic acid (TUDCA), an endogenous neuroprotective bile acid and a metabolic regulator, stimulates NSC proliferation and enhances adult NSC pool in vitro and in vivo. In this study, we dissected the mechanism triggered by this proliferation-inducing molecule, namely in mediating metabolic reprogramming. Liquid chromatography coupled with mass spectrometry based detection of differential proteomics revealed that TUDCA reduces the mitochondrial levels of the long-chain acyl-CoA dehydrogenase (LCAD), an enzyme crucial for β-oxidation of long-chain fatty acids (FA).

INSTRUMENT(S): maXis

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Neuronal Stem Cell

SUBMITTER: Ana Varela Coelho

LAB HEAD: Ana Varela Coelho

PROVIDER: PXD017979 | Pride | 2021-09-09

REPOSITORIES: Pride

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Reprogramming of Lipid Metabolism as a New Driving Force Behind Tauroursodeoxycholic Acid-Induced Neural Stem Cell Proliferation.

Fernandes Marta B MB Costa Márcia M Ribeiro Maria Filipe MF Siquenique Sónia S Sá Santos Sónia S Martins Joana J Coelho Ana V AV Silva Margarida F B MFB Rodrigues Cecília M P CMP Solá Susana S

Frontiers in cell and developmental biology 20200526

Recent evidence suggests that neural stem cell (NSC) fate is highly dependent on mitochondrial bioenergetics. Tauroursodeoxycholic acid (TUDCA), an endogenous neuroprotective bile acid and a metabolic regulator, stimulates NSC proliferation and enhances adult NSC pool <i>in vitro</i> and <i>in vivo</i>. In this study, we dissected the mechanism triggered by this proliferation-inducing molecule, namely in mediating metabolic reprogramming. Liquid chromatography coupled with mass spectrometry (LC- ...[more]

PMID: 32582686

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