Proteomics

Dataset Information

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Phosphoproteome analysis of signaling by ErbB2 in ovarian cancer cell line (2nd approach)

ABSTRACT: The gene for receptor tyrosine kinase ErbB2 is amplified in breast and ovarian tumours. The linear pathway by which signals through ErbB2 are transduced is well known. However, second generation questions that address spatial aspects of signaling remain. To address this, we have undertaken a mass spectrometry approach to identify phosphoproteins specific for ErbB2 using the inhibitors Lapatinib and CP724714 using TIO2 and nano-LC-ESI MS/MS

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Sidhanth Jayprakash  

LAB HEAD: Dr.T.S. Ganesan

PROVIDER: PXD017985 | Pride | 2025-12-29

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
01102019_SIDDHANT_CP_ETD.mgf Mgf
01102019_SIDDHANT_CP_ETD.pride.mgf.gz Mgf
01102019_SIDDHANT_EGF_ETD.mgf Mgf
01102019_SIDDHANT_EGF_ETD.pride.mgf.gz Mgf
01102019_SIDDHANT_LAP_ETD.mgf Mgf
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Publications

Phosphoproteomic analysis reveals the diversity of signaling behind ErbB-inhibitor-induced phenotypes.

Vaparanta Katri K   Song Zejia Z   Farahani Iman I   Jokilammi Anne A   Merilahti Johannes J   Örling Johanna J   Virtanen Noora N   Haapaniemi Pekka P   Sahlgren Cecilia C   Elenius Klaus K   Paatero Ilkka I  

The FEBS journal 20250724 24

The impact of kinase inhibitors on the phosphoproteome has been rarely investigated at a whole-organism level. Here, we performed a phosphoproteomic analysis in embryonic zebrafish to identify the signaling pathways perturbed by ErbB receptor tyrosine-protein kinase inhibitors gefitinib, lapatinib, and AG1478 at the organism level. The phosphorylation of proteins associated with the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt), p38 mitogen-activated protein kinase (MAPK), Notch, Hippo  ...[more]

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