K-562 GalNAc-T1/2 Bump-and-hole Whole cell lysate proteomics data

ABSTRACT: Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAc analog into the glycoproteins on the cell surface of K-562 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-azide. Glycoproteins were enriched using Streptavidin, and on-bead digestion yielded a peptide fraction that was analysed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Benjamin Schumann  

LAB HEAD: Benjamin Schumann

PROVIDER: PXD017989 | Pride | 2020-04-22

REPOSITORIES: Pride