Dataset Information

Multi-omics reveals the lipid inventory of synaptic junctions and altered lipid signaling in environmental enrichment

ABSTRACT: Membrane lipids are essential components of synaptic junctions that have key functions in neurotransmission. In the first part of the study, we provide a quantitative lipid inventory of mouse and rat synaptic junctions. We next used a simultaneous multi-omics extraction and analysis workflow termed SIMPLEX to probe the interplay of proteins and lipids in synaptic signal transduction. We found that SIMPLEX is suitable to generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach revealed that in mice exposed to an enriched environment reduced endocannabinoid signaling is linked to increased surface expression of AMPAR in a subset of Cannabinoid-receptor 1 positive synapses, a mechanism which likely regulates synaptic strength in an input-specific manner.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Canan Has

LAB HEAD: Robert Ahrends

PROVIDER: PXD017997 | Pride | 2023-11-14

REPOSITORIES: Pride

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Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.

Borgmeyer Maximilian M Coman Cristina C Has Canan C Schött Hans-Frieder HF Li Tingting T Westhoff Philipp P Cheung Yam F H YFH Hoffmann Nils N Yuanxiang PingAn P Behnisch Thomas T Gomes Guilherme M GM Dumenieu Mael M Schweizer Michaela M Chocholoušková Michaela M Holčapek Michal M Mikhaylova Marina M Kreutz Michael R MR Ahrends Robert R

Cell reports 20211001 1

Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proo ...[more]

PMID: 34610315

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	F058132.dat	Other
	Lip06_0806_globalno_normalization.msf	Msf
	Lumos00330.raw	Raw
	Lumos00331.raw	Raw
	Lumos00332.raw	Raw