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MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity

ABSTRACT: The interaction partners of C. elegans soluble IL-17 signaling components were profiled using IP/MS. In vivo interactions were profiled for ACTL-1-FLAG, PIK-1-Myc, MALT-1::GFP, NFKI-1::GFP and their respective controls. Quantification of NFKI-1::GFP interactions was performed by labelling immunoprecipitated proteins using tandem mass tags.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Mark Skehel

LAB HEAD: Mario de Bono

PROVIDER: PXD018000 | Pride | 2020-05-27

REPOSITORIES: Pride

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MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity.

Flynn Sean M SM Chen Changchun C Artan Murat M Barratt Stephen S Crisp Alastair A Nelson Geoffrey M GM Peak-Chew Sew-Yeu SY Begum Farida F Skehel Mark M de Bono Mario M

Nature communications 20200429 1

Besides pro-inflammatory roles, the ancient cytokine interleukin-17 (IL-17) modulates neural circuit function. We investigate IL-17 signaling in neurons, and the extent it can alter organismal phenotypes. We combine immunoprecipitation and mass spectrometry to biochemically characterize endogenous signaling complexes that function downstream of IL-17 receptors in C. elegans neurons. We identify the paracaspase MALT-1 as a critical output of the pathway. MALT1 mediates signaling from many immune ...[more]

PMID: 32350248

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