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Shotgun proteomics of Frankia alni grown on fumarate

ABSTRACT: Next-generation proteomics of Frankia alni for defining the key proteins involved in growth on fumarate.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Frankia Alni

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD018045 | Pride | 2023-06-11

REPOSITORIES: Pride

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Frankia alni Carbonic Anhydrase Regulates Cytoplasmic pH of Nitrogen-Fixing Vesicles.

Pujic Petar P Carro Lorena L Fournier Pascale P Armengaud Jean J Miotello Guylaine G Dumont Nathalie N Bourgeois Caroline C Saupin Xavier X Jame Patrick P Selak Gabriela Vuletin GV Alloisio Nicole N Normand Philippe P

International journal of molecular sciences 20230523 11

A phyloprofile of Frankia genomes was carried out to identify those genes present in symbiotic strains of clusters 1, 1c, 2 and 3 and absent in non-infective strains of cluster 4. At a threshold of 50% AA identity, 108 genes were retrieved. Among these were known symbiosis-associated genes such as nif (nitrogenase), and genes which are not know as symbiosis-associated genes such as can (carbonic anhydrase, CAN). The role of CAN, which supplies carbonate ions necessary for ca ...[more]

PMID: 37298114

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