Dataset Information

The interactome of BTN3A1 (Butyrophilin Subfamily 3 Member A1)

ABSTRACT: In this study we dissected the BTN3A1 interactome before or after the treatment with pamidronate to gain mehcanistic insights into the gamma9/delta2 T cell dependent recognition of target cells. Our approach relied on affinity purification couple with mass spectrometry from a BTN3A knock-out cell line stably reconstituted with FLAG-BTN3A1. Our findings revealed an important role of cellular trafficking of BTN3A1 in the gamma9/delta2 TCR-induced T cell activation.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Domenico Fasci

LAB HEAD: Albert J. R. Heck

PROVIDER: PXD018055 | Pride | 2020-06-05

REPOSITORIES: Pride

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Publications

γ9δ2T cell diversity and the receptor interface with tumor cells.

Vyborova Anna A Beringer Dennis X DX Fasci Domenico D Karaiskaki Froso F van Diest Eline E Kramer Lovro L de Haas Aram A Sanders Jasper J Janssen Anke A Straetemans Trudy T Olive Daniel D Leusen Jeanette J Boutin Lola L Nedellec Steven S Schwartz Samantha L SL Wester Michael J MJ Lidke Keith A KA Scotet Emmanuel E Lidke Diane S DS Heck Albert Jr AJ Sebestyen Zsolt Z Kuball Jürgen J

The Journal of clinical investigation 20200901 9

γ9δ2T cells play a major role in cancer immune surveillance, yet the clinical translation of their in vitro promise remains challenging. To address limitations of previous clinical attempts using expanded γ9δ2T cells, we explored the clonal diversity of γ9δ2T cell repertoires and characterized their target. We demonstrated that only a fraction of expanded γ9δ2T cells was active against cancer cells and that activity of the parental clone, or functional avidity of selected γ9δ2 T cell receptors ( ...[more]

PMID: 32484803

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Project description:While the majority of protein-coding genes in the human genome have been identified, the location of the regulatory sequences that control their expression in time and space remains poorly defined. The importance of identifying these elements is underscored by a growing number of studies (such as GWAS) supporting a relationship between variation in non-coding sequences and human disease. M-BM- emonstrated that ChIP-Seq with p300 directly on human or mouse tissues represents a powerful method to identify the location and tissue-specific activity pattern of enhancers in the genome.M-BM- a highly specific chromatin mark for the prediction of in vivo enhancers, it is not without limitations. M-BM- comparison of p300-bound regions with previously generated enhancer sets shows that the majority of enhancers are activated independently of p300. To attempt to identify a larger proportion of enhancers in vivo, we have focused on several additional transcriptional coactivators that are hypothesized to be associated with active tissue-specific enhancers. M-BM- limited availability of ChIP-seq grade antibodies for these coactivators, FLAG-tag knock-in mice were generated and ChIP-seq using a FLAG antibody was performed on various tissues from e11.5 mouse embryos. Indeed, as these mouse lines have become available we have validated their valuable role as marks for transcriptional enhancers in vivo.M-BM- studies are expected to further expand the catalogue of in vivo functional enhancers, which will aid in the decoding of the regulatory genome and provide new insights into the role of gene regulation in human biology and disease. In addition to FLAG data, this accession includes histone acetylation / methylation ChIP-seq data from e11.5 mouse embryonic tissues. Histone ChIP-seq data was used in combination with FLAG data for various computational analyses. Examination of FLAG-labeled transcriptional coactivator binding in mouse embryonic stage 11.5 and embryonic stem cells

Dataset Information

The interactome of BTN3A1 (Butyrophilin Subfamily 3 Member A1)

Publications

γ9δ2T cell diversity and the receptor interface with tumor cells.

Similar Datasets

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