Dataset Information

Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers

ABSTRACT: Intracellular traffic between compartments of the secretory and endocytic pathways is mediated by vesicle-based carriers. The proteomes of carriers destined for many organelles are ill-defined because the vesicular intermediates are transient, low-abundance and difficult to purify. Here, we combine vesicle relocalisation with organelle proteomics and Bayesian analysis to define the content of different endosome-derived vesicles destined for the trans-Golgi network (TGN). The golgin coiled-coil proteins golgin-97 and GCC88, shown previously to capture endosome-derived vesicles at the TGN, were individually relocalised to mitochondria and the content of the subsequently re-routed vesicles was determined by organelle proteomics. Our findings reveal 45 integral and 51 peripheral membrane proteins re-routed by golgin-97, evidence for a distinct class of vesicles shared by golgin-97 and GCC88, and various cargoes specific to individual golgins. These results illustrate a general strategy for analysing intracellular sub-proteomes by combining acute cellular re-wiring with high-resolution spatial proteomics

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Kathryn Lilley

LAB HEAD: Sean Munro

PROVIDER: PXD018110 | Pride | 2020-12-01

REPOSITORIES: Pride

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Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.

Shin John J H JJH Crook Oliver M OM Borgeaud Alicia C AC Cattin-Ortolá Jérôme J Peak-Chew Sew Y SY Breckels Lisa M LM Gillingham Alison K AK Chadwick Jessica J Lilley Kathryn S KS Munro Sean S

Nature communications 20201125 1

Intracellular traffic between compartments of the secretory and endocytic pathways is mediated by vesicle-based carriers. The proteomes of carriers destined for many organelles are ill-defined because the vesicular intermediates are transient, low-abundance and difficult to purify. Here, we combine vesicle relocalisation with organelle proteomics and Bayesian analysis to define the content of different endosome-derived vesicles destined for the trans-Golgi network (TGN). The golgin coiled-coil p ...[more]

PMID: 33239640

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Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers

Publications

Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.

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